Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.) Filing date Publication date Application filed by Heilongjiang University filed Critical Heilongjiang University Priority to CN 201110318877 priority Critical patent/CN102344898B/en Publication of CN102344898A publication Critical patent/CN102344898A/en Application granted granted Critical Publication of CN102344898B publication Critical patent/CN102344898B/en Status Expired - Fee Related legal-status Critical Current Anticipated expiration legal-status Critical Links Original Assignee Heilongjiang University Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.) ( en Inventor 赵凯 张小燕 郝妍 常志威 孙立庆 夏昕 Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.) Granted Application number CN2011103188771A Other languages Chinese ( zh) Google Patents CN102344898A - Klebsiella variicola strain HgCl2 does not give white precipitate with aminoacids, so the microorganism which hydrolyses gelatin give no white precipitation with HgCl2.CN102344898A - Klebsiella variicola strain These aminoacids are then utilizes by microorganisms. The microorganism producing an extracellular gelatinase hydrolyses gelatin releasing constituent aminoacids and short peptides. HgCl2 forms white precipitate with gelatin protein. The presence of gelatin protein is detected by adding acidic HgCl2 (mercuric chloride). Gelatin is polymer of aminoacids linked by peptide bonds. Once the gelatin is hydrolysed, even 4☌ temperature cannot restore gel like characteristic. Gelatin dissolves in water at 50☌ and exist in liquid form above 25☌ and solidifies as gel below 25☌. Collagen is the major component of connective tissues and tendon I humans and animals. Gelatin is a globular protein that is produced by hydrolysis of collagen. The main purpose of this test is to detect the ability of organism to produce enzyme gelatinase. to test whether the organism produces gelatin hydrolyzing enzyme gelatinase or not.
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