![]() ![]() Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.) Filing date Publication date Application filed by Heilongjiang University filed Critical Heilongjiang University Priority to CN 201110318877 priority Critical patent/CN102344898B/en Publication of CN102344898A publication Critical patent/CN102344898A/en Application granted granted Critical Publication of CN102344898B publication Critical patent/CN102344898B/en Status Expired - Fee Related legal-status Critical Current Anticipated expiration legal-status Critical Links Original Assignee Heilongjiang University Priority date (The priority date is an assumption and is not a legal conclusion. ![]() Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.) ( en Inventor 赵凯 张小燕 郝妍 常志威 孙立庆 夏昕 Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.) Granted Application number CN2011103188771A Other languages Chinese ( zh) Google Patents CN102344898A - Klebsiella variicola strain HgCl2 does not give white precipitate with aminoacids, so the microorganism which hydrolyses gelatin give no white precipitation with HgCl2.CN102344898A - Klebsiella variicola strain These aminoacids are then utilizes by microorganisms. The microorganism producing an extracellular gelatinase hydrolyses gelatin releasing constituent aminoacids and short peptides. HgCl2 forms white precipitate with gelatin protein. The presence of gelatin protein is detected by adding acidic HgCl2 (mercuric chloride). ![]() Gelatin is polymer of aminoacids linked by peptide bonds. Once the gelatin is hydrolysed, even 4☌ temperature cannot restore gel like characteristic. Gelatin dissolves in water at 50☌ and exist in liquid form above 25☌ and solidifies as gel below 25☌. Collagen is the major component of connective tissues and tendon I humans and animals. Gelatin is a globular protein that is produced by hydrolysis of collagen. The main purpose of this test is to detect the ability of organism to produce enzyme gelatinase. to test whether the organism produces gelatin hydrolyzing enzyme gelatinase or not.
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The bracket attaches to the Jeep JK using factory bolts. Complete HD Skid Plate System Includes: Oil Pan Skid, Transmission Skid, & Transfer Case Skid. Item J101361 ExtremeTerrain no longer carries the Synergy Manufacturing Oil Pan Skid Plate Black (07-11 Jeep Wrangler JK). Bracket will ship bare metal and include wires, 4 butt connectors and 6mm metric bolts to bolt the vacuum pump to our bracket. Synergy Jeep JK Heavy Duty Complete Skid Plate System fits 12-Present Jeep Wrangler JK and Wrangler Unlimited JK. The bracket is CNC cut from 11 gauge steel plate and features the Motobilt Logo. We have designed a bracket for the Jeep JK 2012-present that relocates your vacuum pump to under the hood in the engine compartment. When mounting an aftermarket bumper or winch on your Jeep JK or JK Unlimited it may be necessary to relocate the factory vacuum pump from the front cross - member. Motobilt offers a Jeep JK Vacuum Pump Relocation Bracket. To reflect the policies of the shipping companies we use, all weights will be rounded up to the next full pound.Product Description Motobilt Vacuum Pump Relocation Bracket (Wrangler JK 2007-2018) In addition, this skid plate system must be used with 2+ in. We manufacture the toughest skid plates to protect your transfer case, oil pan. Though this product is not compatible with diesels, it will accommodate all Synergy stage 4 long arm kits. The weight of any such item can be found on its detail page. This stellar skid plate system comes with the oil pan skid, transmission skid, and the transfer case skid. These are manufactured from 3/16 crush resistant Grade 50 A-572 steel plate to reduce weight while not sacrificing strength. Synergy Manufacturing 1.75' Hub Centric Wheel Spacer, 5X4.5 Bolt Pattern, 1/2-20 UNF Stud Size - Pair. Please also note that the shipping rates for many items we sell are weight-based. Oil Pan Skid Plate (2) Parking Brake Hardware Kit (1). Ship to Home: Ships Directly From Manufacturer Timing based on Manufacturer's availability. Made from 3/16 Crush-Resistant Steel Cut Using CNC Technique Comes with Integrated Mounting Brackets Comes with Grade 8 Hardware Allows Access to Drain. Depending on the shipping provider you choose, shipping date estimates may appear on the shipping quotes page. Synergy Manufacturing Oil Pan Skid Plate (Black) - 5710-04-BK. When you place an order, we will estimate shipping and delivery dates for you based on the availability of your items and the shipping options you choose. Note that there are restrictions on some products, and some products cannot be shipped to international destinations. Fab Fours Transmission and Oil Pan Skid Plate for 07-18 Jeep Wrangler JK. ARB Engine Oil Pan Skid Plate for 12-18 Jeep Wrangler JK with 3.6L Engine. ![]() We can ship to virtually any address in the world. ARB Under Vehicle Skid Plates for 07-11 Jeep Wrangler JK with 3.8L. We'll notify you via e-mail of your refund once we've received and processed the returned item. If you need to return an item, simply login to your account, view the order using the 'Complete Orders' link under the My Account menu and click the Return Item(s) button. This time period includes the transit time for us to receive your return from the shipper (5 to 10 business days), the time it takes us to process your return once we receive it (3 to 5 business days), and the time it takes your bank to process our refund request (5 to 10 business days). Synergy Manufacturing HD Skid Plate System (Black) - 5710-BK 5. ![]() You should expect to receive your refund within four weeks of giving your package to the return shipper, however, in many cases you will receive a refund more quickly. We'll also pay the return shipping costs if the return is a result of our error (you received an incorrect or defective item, etc.). Designed to be welded on the front or rear Jeep JK lower control arm mounts. You may return most new, unopened items within 30 days of delivery for a full refund. Jeep JK D30/35/44 Lower Control Arm Skid Plate. ![]() According to Ben Blackwell, Third Man’s cofounder and head of its vinyl operation, manufacturing the little black records in the digital era requires extra diligence. Since then the label has released just over 300 7-inch singles. A new Sub Pop batch, the first in a decade, arrives next month.Ĭontinuing his attachment to vinyl formats, Jack White revived the 45 on his Third Man label, starting with a Dead Weather single a decade ago. Sub Pop launched its first Singles Club in 1988, initially shipping a monthly 45 to members that included releases by Nirvana, the Flaming Lips and a shared Sonic Youth–Mudhoney venture. The seven-inch never fully recovered, but it nonetheless endures. The number of jukeboxes in the country declined, boomer rock fans increasingly gravitated toward albums, and the cassette format (and even the wasteful “cassette single” and “mini-CD” format) began overtaking vinyl 45s. ![]() By the early Eighties, the 45 began dying a slow, humiliating death. The following decade, indie fans who snapped up Hüsker Dü’s “Makes No Sense at All” found their unlikely but fantastic cover of “Love Is All Around,” otherwise known as the Mary Tyler Moore Show theme song, on the flip.Īccording to the New York Times, the peak year for the seven-inch single was 1974, when 200 million were sold. If you flipped over Fleetwood Mac’s “Go Your Own Way” in 1977, you’d come across “Silver Springs,” the Stevie Nicks landmark that was dumped from Rumours. Some singles had picture sleeves or B sides of outtakes. ![]() For added head-scratching, each 45 was printed in a different color, from “deep red” to “dark blue.” (Yes, colored vinyl actually existed in the years immediately after World War II.) Not quite the stuff of the pop charts at that moment in history. The one most people will remember is Arthur “Big Boy” Crudup’s jumping-bean boogie “That’s All Right,” which became Elvis Presley’s breakout moment in the next decade, but the list also included a Yiddish song, “A Klein Melamedl (The Little Teacher),” sung by a cantor. Then consider those initial seven RCA releases, which, according to the label’s archives, ranged from classical to kids’ music to country. “They’ll come in, ask for a recording, and then ask me whether or not it can be played on the particular phonogram they have at home.” More often than not, he said, potential buyers left without forking over any cash. ![]() “My customers don’t know what to buy anymore,” a record store owner groused to the trade magazine Cashbox that month. The size of 45s alone, combined with the fact that different gear was suddenly required to play them, was enough to perplex the pre-rock music business. On March 15th, 1949, RCA Victor became the first label to roll out records that were smaller (seven inches in diameter) and held less music (only a few minutes a side) than the in-vogue 78s. I will read messages from new contacts, but only periodically due to harassment from chasers, transphobes and smokers.When it arrived 70 years ago today, the 45 rpm single, a format that would revolutionize pop music, seemed less radical than simply confusing. You can find me on facebook, but only accept friend requests from people I know face to face. I'm a lifelong atheist and cynic, but mellowed a bit since transitioning five years ago. I'm a Centennial kid from Canada who moved to Asia permanently in 2001, living in Taiwan since 2005. ![]() There are two ways to check if the custard is ready.
![]() And I think there will be some “wait and see” in the fall from recruits who want to know what kind of team the Tigers will roll out on the field. ![]() But if Freeze and his staff can stabilize the Tigers’ position in the Blue-Chip Ratio and build on that for the 20 classes, they’ll be heading in the right direction. I don’t expect Auburn to land some mega-elite class this cycle, and it would take quite a massive effort to push into top-10 range. Until then, I think it’s still too early to tell. By the time we’re a week or two removed from Big Cat Weekend, which is at the end of the month, we’ll have a good idea of where Auburn is projected on the recruiting trail for the 2024 class. The dead period that starts on Monday should be an active one for commitments, as recruits stop making visits and start making decisions before their senior seasons of high school. That means they were able to bring in elite recruits without it being their final, biggest visit - and they were able to land a guy like Joe Phillips from Georgia’s clutches by just having him for a camp. Now, it’s about getting the numbers.Īuburn had a lot of kids on campus during this most recent recruiting period, and it didn’t use up many of its official visits. That’s a solid foundation, and it’s more like what Auburn should be bringing in year in and year out. The Tigers have eight commitments for 2024, and six of them are blue-chips, with the top two being both top-150 overall players and top-10 players at their respective positions. The early calculations are definitely skewed by volume.) 9 in the SEC, but it’s a class made up of three 5-stars and four 4-stars. ![]() (Alabama is also experiencing some of this - No. But, with an average recruit rating of 90.75, that’s much more in line with a top-half SEC class and a top-15 or so class in the country. I was interested to read an "unnamed" SEC assistant took shots at the Auburn coaching staff this week saying, "Freeze didn't deliver the all-star staff he promised." How would you evaluate Freeze's first Auburn staff so far, knowing they have yet to coach a down of meaningful football?Īuburn’s 2024 class currently sits at No. They started from so far behind, how do you think they are doing making up ground? I know you're not a recruiting guy, but Coach Freeze has said that the 2024 & 2025 classes will make or break his Auburn tenure. Thanks as always for supporting The Auburn Observer, especially during the dog days of the offseason. Toward the end of the mailbag, we’ve got a good question about the 2023-24 Auburn basketball team and a lightning round of bonus questions ranging from alternate uniforms, international recruiting, Weird Al Yankovic and a two-word noun that I didn’t think I would ever write in here. We’ll also talk the impact of facilities and NIL, look at the future of conference title games in the new era and rank some recent schedules from easiest to toughest. This week’s mailbag talks a lot about recruiting and projecting into the future for Freeze and his first staff. ![]() The last seven months have been a big-time turnaround, especially when you throw the transfer portal into the mix, but it’s just the first step in a process to get Auburn back to competing with its elite rivals on college football’s biggest stages. So far, Auburn has done a lot more than the previous staff - but the bar was pretty low there. As I’ve mentioned multiple times in recent newsletters, Hugh Freeze arrived on the Plains and wasted no time in prioritizing the Tigers’ massive need in that department. Specifically, it’s time to talk about Auburn football’s recruiting. After spending a lot of last week’s mailbag looking toward the past, it’s time to talk about the future. ![]() Kory Grow from Rolling Stone praised the use of the " Baby Got Back" sample and said the song stayed true to the theme of the original. Problems playing this file? See media help.Ĭarolyn Menyes from Music Times said it is a bit "raunchy" though this was "expected" and saying "Anaconda" continues Minaj's promise to move away from her poppier singles such as " Super Bass" and " Starships", and go back to her hardcore rap roots. The song is written in the key of C ♯ minor with a tempo of 130 beats per minute. ![]() Musically, the song is a hip hop and pop-rap song. Production team Da Internz were then brought in to add drums to the song, whereafter it was sent for mixing and mastering, which made it "perfect". Īccording to Minaj's sound engineer Aubry "Big Juice" Delaine, "Anaconda" had 27 different versions at first. Whereas Sir Mix-a-Lot focuses on a woman's body and the pleasure it gives him, Minaj raps from the perspective of the unnamed woman, and shows how she uses her body to profit and empower herself. The song has been viewed by some as a diss track, in answer to " Baby Got Back". The song heavily samples " Baby Got Back" by Sir Mix-a-Lot, who also has vocals in the chorus of the song. On various digital music stores, the cover art is censored with the Parental Advisory label over Minaj's buttocks. The cover art for the single went viral on the web after being revealed. However, on August 24, 2014, Elliott contradicted Polow's recollections, saying she never received the instrumental. On August 5, 2014, producer Polow da Don claimed that the "Anaconda" instrumental was originally intended for rapper and musician Missy Elliott, but was scrapped and then re-recorded by Minaj. The song was also nominated for two categories at the 2015 MTV Video Music Awards, Best Female Video and Best Hip-Hop Video, winning the latter. "Anaconda" was nominated for Best Rap Song at the 2015 Grammy Awards. To further promote the song, Minaj performed it live at the 2014 MTV Video Music Awards and also performed it at Fashion Rocks and the iHeartRadio Music Festival. ![]() In 2021, Anaconda became the first music video for a solo female rap song to hit 1 billion views. Following the video's release, it broke the 24-hour streaming record on Vevo by accumulating 19.6 million views in its first day of release. The song also peaked within the top ten in Australia, Canada, Ireland, New Zealand and the United Kingdom, top forty in Austria, France and Portugal and top fifty in Czech Republic.Ĭolin Tilley directed an accompanying video for the song, which was released on August 19, 2014. The song peaked at number two on the US Billboard Hot 100, becoming Minaj's highest charting single at the time. Released on Augby Young Money Entertainment, Cash Money Records, and Republic Records as the second single from the album, the song was produced by Polow da Don, DJ Spydr, and Da Internz, and prominently samples " Baby Got Back" (1992) by Sir Mix-a-Lot. " Anaconda" is a song by rapper Nicki Minaj from her third studio album, The Pinkprint (2014). For other songs with the same title, see Anaconda (disambiguation) § Music. |
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